Composite
OmpR Siali
Part:BBa_K2235007:Design
Designed by: Morgan Hjorth and Louise Larsson Group: iGEM17_Stockholm (2017-10-11)
OmpR promoter regulating the expression of sialidase enzyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 189
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 636
Illegal NgoMIV site found at 711
Illegal NgoMIV site found at 801 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1181
Design Notes
The sialidase contains a HIS-tag at the N-terminal for easy purification.
Source
BBa_R0082 (OmpR from iGEM Antiquity) BBa_K22355006 (RBS sialidase from iGEM Stockholm 2017)